There are 12 teams in total in this tier 4 division in 2025/26, including newly promoted Kidderminster Harriers.

The full line-up is -
Barnsley Women
Boldmere St Michaels
Kidderminster Harriers
Leafield Athletic
Lincoln United
Northampton Town
Notts County
Peterborough United
Sheffield FC
Stourbridge FC
Sutton Coldfield Town
Worcester City

As a result of Blackburn Rovers withdrawing from the WSL2 (at tier two), Sheffield United have been reprieved from relegation and at tier 3, Halifax FC have also been reprieved and will stay in the Northern Premier Division.

Blackburn Rovers have been assigned to tier 4 and been placed in the Division 1 North.